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Microscope photography with the Nikon SMZ1500 stereoscopic zoom microscope

The Nikon SMZ1500 stereoscopic zoom microscope is a very popular instrument, especially in the United States, and is also increasingly in demand in Europe. We tested it extensively with our products. For our tests we used two different cameras: a Nikon D4 full-frame camera and a Canon EOS 650D with APS-C sensor. The Canon EOS 650D (see left-hand picture) was connected to the microscope using a TUST38C (C-mount 1x port) and a DSLRCTCW_Pro wide-field adapter. To mount the Nikon D4 (see right-hand picture) to the Nikon SMZ1500, we used a Nikon C-mount TV adapter A (as an alternative to our TUST38C) and our DSLRNFC adapter. 

Nikon SMZ1500 with beam splitter

Nikon SMZ 1500 beam splitter with Nikon D4 and DSLRNFC adapter     

It is said that first impressions count, and our first impressions were very good. The Nikon SMZ1500 stereoscopic microscope is designed according to the telescope principle (Abbe principle). It is constructed from very high-grade materials and comes with a solid, stable stand. Furthermore, it has both a coarse and a fine coaxial focussing unit, which prevents backlash. Thanks to the large working distance (at high magnifications up to 50 mm), it also offers enough room for illumination and working. Its adjustable eyepiece tube (angle of incline from 0º to 30º) and low eye level make it very comfortable to work with. In addition, the eyepieces are equipped with built-in diopter adjustment.

Nikon SMZ1500 coarse and fine focussing

For our test series, we used the HR Plan Apo 1x and the standard C-W10x eyepiece with a field of view diameter of 22 mm. This combination yielded total magnifications of 7.5x to 112.5x. The resulting image field is very large and bright.

Nikon SMZ 1500 lens

The optical quality of the Nikon SMZ1500 is excellent. With stereoscopic microscopes there are often field curvature issues and chromatic aberrations (colour fringing). But Nikon has solved this problem, with the images appearing flat and with no aberrations (see butterfly wing). Even with histological slides, the microscope delivers an excellent performance (see picture below).

no aberrations butterfly wing with Nikon SMZ1500  no aberrations histological slide with Nikon SMZ 1500

The built-in aperture diaphragm is very useful for adjusting the optimum depth of field.

Nikon SMZ stereo microscope built-in aperture diaphragm

The zooming knob is very easy to operate. The microscope has an above-average zoom range of 0.75 to 11.25x. Lower priced entry models mostly only offer a zoom factor of 6x to a maximum of 10x. This selector knob eliminates the need to remove your eyes from the eyepiece while changing magnification.

Photography of smallest details Zooming knob Nikon SMZ1500

The Nikon SMZ1500 can be very easily fitted with a digital SLR or system camera (mirrorless with interchangeable lens mounts). Digital SLR cameras offer very high quality and have an excellent price-performance ratio. Using them enables the optical potential of this microscope to be utilised very well. 

double beam path Nikon SMZ1500

A factor 1x C-mount port (without built-in lens) is mounted directly onto the beam splitter, and the LM DSLR adapter, together with the DSLR camera body, is then screwed onto the port. When choosing the ideal camera, you would do well to refer to our camera rankings for guidance. Especially at low magnifications (up to 20x), full-frame cameras (e.g. the Nikon D4, Nikon D800, or Canon 5D Mark III) achieve very good results. Nevertheless, cameras with smaller sensors (e.g. the Canon EOS 650D or the Nikon D7000) also deliver a very decent image quality. 

Photography Magnification changer Nikon SMZ1500

The magnification changer for using different lenses is a very convenient accessory.

magnification changer for Nikon SMZ1500

For photomicrographic images, the lens can be tilted. The lens can be positioned centric to the optical path, exactly where the camera is mounted. As a result, the area close to the axis of the plan apo lens is used, which is a prerequisite for focus stacking (z-stack). At high magnifications, in particular, the depth of field is very small due to physical limitations. For this reason, for three-dimensional slides, we recommend that you purchase a motorised version. This makes it possible to capture individual images at different levels that can then be combined using a special software program (e.g. Helicon, CombineZ) to produce a single image with an enormous focal range. The image below, for example, shows a butterfly wing magnified 125x its original size.

butterfly wing magnified 125x focus stacking

Conclusion: Working with the Nikon SMZ1500 is definitely very convenient. The advantages are obvious: it is very stable and well-built, offers a large zoom range (with one lens!), has a high degree of planarity with no image flaws and a large working distance. In addition, lots of accessories are available for purchase. The disadvantages of the Nikon SMZ1500 are its relatively high price and the fact that, due to the construction type (Abbe principle), it provides only a limited three-dimensional impression.

14.01.2013


Photography

Fitting the microscope to digital single-lens reflex (DSLR), system or C-mount cameras is easy with our LM digital SLR adapters, which feature a plan achromatic optical system. Our products make it possible to capture top-quality microscope images. To help you select the adapter that is right for your camera, we have set up an online configurator on our website. You can also email us – ideally with attached photographs of your microscope.

Modern DSLR and system cameras offer the latest technology and are generally very well suited for microscopy applications. Most of them can be controlled remotely via PC/Mac. Because of their high sales volumes, they offer an excellent price/performance ratio compared to special-purpose microscope cameras.

Features of top DSLR and system cameras:
- Large, powerful full-frame sensors (36 x 24 mm)
- Sensor resolution of 50 megapixels
- High light sensitivity (ISO 100,000+)
- Extensive dynamic range (up to 14 aperture stops/f-stops)
- Short exposure times (1/8000 second):
- Full/Ultra HD (4k) video function
- Live video capture on external monitors in ultra HD quality

In most cases, these cameras are significantly more powerful than microscope cameras with smaller sensors (1/2" or 2/3"). On our website you will find our current camera recommendations and a camera ranking which is specifically tailored to microscopy applications.
 




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