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Nikon Eclipse E400 and E600

Nikon’s Eclipse microscopes were introduced to the market in the 1990s (the E400 in 1997 and the E600 in 1990). Both feature an infinity-corrected optical system and were designed for “workhorse” applications in clinical research.

All current DSLR and system cameras can be attached to Nikon’s Eclipse series microscopes quickly and easily using LM digital adapters, which results in a maximum of image quality.

Nikon Eclipse E400 adapter solution laboratory microscope

Nikon advertised its E400, which represents the mid-level price segment, as the “8-hour microscope” due to its excellent ergonomics and progressive design: all control knobs in a single line, new focussing system, low-profile stage and practical positioning of epi-fluorescence filters. Although the E400 was designed with stability in mind, it nevertheless features a compact design.

The E400’s optical system features Nikon’s acclaimed CFI-60 lenses, which offer the combined advantages of greater working distances and a higher numerical aperture. The series of universal objectives included with the system enable the use of various observation techniques (bright field, dark field, Nomarski DIC, epi-fluorescence or phase contrast) without the need to change objectives. The E400 features a quintuple revolving nosepiece, and the magnification capability of its optical system ranges from 10x to 1,500x for observation purposes.

The E600, another mid-tier instrument, also comes equipped with the CFI-60 optical system mentioned above, and users can opt for either a sextuple revolving nosepiece or a sextuple DIC nosepiece. The infinity-corrected optical system with a parallel optical path enables the use of a variety of components, such as filters, compensators, DIC prisms and reflectors.

Illumination on the E600 model is provided by a 12-volt, 100-watt halogen lamp, while the E400 comes with a 6-volt, 20-watt halogen lamp.

Nikon offers three tube types for its higher-grade Eclipse series microscopes (E400, E600 and E800). The key to properly attaching a DSLR or system camera to a microscope with our high-quality LM digital adapters is to choose the correct combination:

Nikon YT TV Tubus

The following considerations need to be taken into account:

There are many tube types - here are three of them: 

V-TP Photo System Head V-TE Tilting Trinocular TubeV-TF Trinocular Tube

All of our digital adapters are equipped with a plan-achromatic optical system that ensures a flat field of view with no loss of focus at the edges. Since we correct the lenses of our LM digital adapters, they deliver images that are not affected by vignetting, chromatic aberrations (colour fringing) or barrel-shaped distortions. Additionally, they are equipped with an anti-reflex system that prevents glare, reflections and hot spots.

Auxiliary modules are available for both the E400 and E600 models, including an epi-fluorescence module, intermediate tube, eye-level raiser and others.


Fitting the microscope to digital single-lens reflex (DSLR), system or C-mount cameras is easy with our LM digital SLR adapters, which feature a plan achromatic optical system. Our products make it possible to capture top-quality microscope images. To help you select the adapter that is right for your camera, we have set up an online configurator on our website. You can also email us – ideally with attached photographs of your microscope.

Modern DSLR and system cameras offer the latest technology and are generally very well suited for microscopy applications. Most of them can be controlled remotely via PC/Mac. Because of their high sales volumes, they offer an excellent price/performance ratio compared to special-purpose microscope cameras.

Features of top DSLR and system cameras:
- Large, powerful full-frame sensors (36 x 24 mm)
- Sensor resolution of 50 megapixels
- High light sensitivity (ISO 100,000+)
- Extensive dynamic range (up to 14 aperture stops/f-stops)
- Short exposure times (1/8000 second):
- Full/Ultra HD (4k) video function
- Live video capture on external monitors in ultra HD quality

In most cases, these cameras are significantly more powerful than microscope cameras with smaller sensors (1/2" or 2/3"). On our website you will find our current camera recommendations and a camera ranking which is specifically tailored to microscopy applications.

New LM Digital Adapter for: Sony Alpha 9 II (ILCE-9M2) / Sony Alpha 9 / Nikon D6 / Sony Alpha 7R IV / Sony Alpha 7S II / Sony Alpha 7R III / Nikon Z6 / Sony Alpha 7R II / Nikon Z7 / Canon EOS R with Canon Adapter EF-EOS R / Canon EOS Ra (Astro) with Canon Adapter EF-EOS R / Canon EOS RP with Canon Adapter EF-EOS R / Sony Alpha 7S / Canon EOS 1D X Mark III / Nikon Z50 / Nikon D850 / Canon EOS 1D X Mark II / Nikon D780 / Sony Alpha 7III / Nikon D5 / Sony Alpha 6600 / Sony Alpha 6400 / Sony Alpha 6100 / Canon EOS 1D X / Nikon D4s / Olympus OM-D E-M5 III / Canon EOS 90D / Canon EOS 5D Mark IV / Nikon D4 / Nikon D750 / Canon EOS 6D Mark II / Fujifilm X-T4 / Fujifilm X-T3 / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Nikon D810 / Nikon D800 / Canon EOS M6 Mark II / Nikon D800E / Nikon Df / Panasonic Lumix DC-G9 / Nikon D610 / Nikon D600 / Canon EOS 250D / Canon EOS 850D / Rebel T8i / Canon EOS 6D / Sony Alpha 99 II (SLT-A99 II) / Canon EOS M200 / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Canon EOS M50 / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Pentax K-1 Mark II / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Olympus OM-D E-M5 II / Nikon D3x / Olympus OM-D E-M1 / Pentax KP / Nikon D3S / Canon EOS 750D / Rebel T6i / Canon EOS 760D / Rebel T6s / Canon EOS 5D Mark II / Nikon D7100 / Pentax K-5 / Canon EOS 1D Mark IV / Nikon D7000 / Canon EOS 7D Mark II / Canon EOS 600D / Rebel T3i / Canon EOS 650D / Rebel T4i / Canon EOS 700D / Rebel T5i / Canon EOS 2000D / Rebel T7 / Canon EOS 7D / Canon EOS 550D / Rebel T2i / Kiss X4 Digital / Canon EOS 1300D / EOS Rebel T6 / Canon EOS 4000D / Canon EOS 100D / Nikon DS-Ri2 (Microscope Camera) / Canon EOS 50D / Canon EOS 1200D / EOS Rebel T5 / EOS Kiss X70 / Canon EOS 1100D / Rebel T3 / Olympus E-5 / Sony Alpha 68 / Panasonic Lumix DMC-GH4 / Canon EOS 1D Mark III / Canon EOS 40D / Canon EOS 60Da for astrophotography / Nikon D7500 / Sony Alpha 99 (SLT-A99) / Sony Alpha 7II /