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An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Online configurator: LM microscope adapter for all digital cameras and microscopes
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Microscope Digital Cameras: Camera ranking for microscopy use

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Sensor heating in Nikon DSLR cameras in Live View mode

The impact of sensor heating on image noise, and thus on image quality, cannot be denied. We wanted to take a closer look at this physical phenomenon and document it.

thermal image of Nikon fullframe sensor in Live view mode without lens

The image above shows the thermal image of a Nikon full frame sensor in Live View mode without lens.

Every electronic device warms up during operation. This is of special interest in digital cameras as there is a connection between image quality and camera temperature. For some applications it definitely makes sense to use cooled camera systems to achieve the best image quality. The signal-to-noise ratio, for example, plays an important role in fluorescence microscopy. In this special form of microscopy, only a small amount of light reaches the camera sensor. For such types of application, camera sensors deliver the best results at cool temperatures.

In the case of photographic documentation, it may very well happen that cameras are running over an extended period of time, especially in poor light conditions, when long exposure times and high ISO settings are required. The images created appear grainy, and tiny coloured dots or specks are visible, particularly in the darker areas. These effects are the result of the camera’s sensor heating up.

We wanted to document sensor heating by using several Nikon cameras (Nikon DF, Nikon D5300, Nikon D610) and therefore took measurements over the course of an hour with a thermal camera. The cameras were set to Live View mode, which is the standard mode in microscopy, and had no lenses attached. If a lens is used, the sensor becomes even hotter than our measurements report.

Temperature increase of the sensor in °C in Live View mode within an hour:

Temperature increase of sensor on C in Live view mode

The curve shows very clearly that the temperature continuously increases over the course of one hour of operation. At the outset, the sensor showed a temperature of 21°C; after an hour, this had already increased to 41.7°C. The sharpest spike in temperature occurred within the first 15 minutes (by 12.6°C). We measured the increase in the temperature of the camera’s sensor in Live View mode. In this mode, the camera uses a lot of power because the integrated electronics are busy processing the data transmitted to the digital signal processor (DSP) in order to transfer the Live View image to the camera display.

thermal camera measuring sensor temperature

Camera manufacturers continuously improve the sensor quality of their cameras in order to minimise the impact of image noise at room temperature and achieve a better signal-to-noise ratio. These efforts are reflected in the ever increasing ISO ranges. ISO values in excess of 200,000 are no longer a rarity. Nevertheless, the correlation between sensor heating and image noise remains a challenge, especially in extreme physical situations, where each photon is to be captured.

In the field of “life science imaging”, for example, fluorescence microscopy places the highest demands on cameras by capturing images of cell cultures, tissues marked with fluorescent dyes, or genetically altered insects that glow of their own accord.

Up until just a few years ago, almost all high-end cameras had to be cooled to reduce the impact of image noise. Several astrophotography enthusiasts even retrofitted commercially available DSLR cameras with a cooling system that reduced the sensor temperature to far below the freezing point.

Conclusion:

Especially when it comes to capturing images in extreme physical situations, it is advisable to refrain from using the Live View mode and to select the lowest ISO settings possible. Furthermore, the user should make sure that the camera is cool (room temperature or below) when working with it. If the camera has already heated up, it should be turned off and only turned back on after it has returned to room temperature. In “everyday” photography, no precautions are necessary, as the influence of sensor heating on image quality is very low.

28.07.2014



New LM Digital Adapter for:

Nikon D850 / Sony Alpha 7III / Sony Alpha 9 / Nikon D5 / Sony Alpha 7S II / Sony Alpha 7R II / Sony Alpha 7R III / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Nikon D610 / Nikon D600 / Canon EOS 6D / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Nikon D3x / Olympus OM-D E-M1 / Nikon D3S / Canon EOS 750D / Rebel T6i / Canon EOS 760D / Rebel T6s / Canon EOS 5D Mark II / Nikon D7100 / Pentax K-5 / Canon EOS 1D Mark IV / Nikon D7000 / Canon EOS 7D Mark II / Canon EOS 600D / Rebel T3i / Canon EOS 650D / Rebel T4i / Canon EOS 700D / Rebel T5i / Canon EOS 2000D / Rebel T7 / Canon EOS 7D / Canon EOS 550D / Rebel T2i / Kiss X4 Digital / Canon EOS 1300D / EOS Rebel T6 / Canon EOS 4000D / Canon EOS 100D / Nikon DS-Ri2 (Microscope Camera) / Canon EOS 50D / Canon EOS 1200D / EOS Rebel T5 / EOS Kiss X70 / Canon EOS 1100D / Rebel T3 / Olympus E-5 / Panasonic Lumix DMC-GH4 / Canon EOS 1D Mark III / Canon EOS 40D / Canon EOS 60Da for astrophotography / Sony SLT-A99 / Sony Alpha 7II / Pentax K-3 II / Olympus E-3 / Olympus E-30 / Olympus E-620 / Sony Alpha 6000 / Pentax K-500 / Canon EOS M100 / Canon EOS M6 / Nikon D700 / Nikon D3 / Canon EOS M5 / Sony Alpha 77V / Canon EOS 1000D / Digital Rebel XS / Sony Alpha 580 / Sony Alpha 5100 / Canon EOS 1Ds Mark III / Sony Alpha 5000 / Pentax K-S2 / Pentax K-r / Sony Alpha 55 / Olympus Pen E-PL7 / Olympus OM-D E-M5 / Olympus OM-D E-M10 / Panasonic Lumix DMC-GX7 / Pentax K-5 II / Pentax K-5 IIs / Pentax K-3 / Sony Alpha 33 / Nikon D5500 / Nikon D5200 / Nikon D5300 /

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High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
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Special mounting media  for microscopy

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