|Micro Tech Lab Professional solutions for digital micro and macro photography|
|An overview of the LM microscope adapters|
|We offer LM digital adapter solutions for the following microscopes|
|Online configurator: LM microscope adapter for all digital cameras and microscopes|
|LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise|
|LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise|
|LM photo microscopes: the flexible photography solution for large sensor cameras!|
|Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index|
|Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo|
|Microscope Digital Cameras: Camera ranking for microscopy use|
Digital photography using the Nikon Alphaphot 2 microscope
The Nikon Alphaphot 2 is available in four different designs (YS2-HF, YS2-H, YS2-TF and YS2-T). The options described here for connecting the microscope to a camera apply to all four models.
The Nikon Alphaphot 2 is a lower-cost, 1990s-era laboratory microscope that features a finite optical system. The CF lenses (the standard is a quadruple revolver: 4x, 10x, 40x and 100x) with which it is equipped, exhibit a very low degree of chromatic aberrations. In general, the microscope is both extremely sturdy and very well manufactured. The condenser, an Abbe type, additionally features an aspheric lens. The built-in aperture diaphragm allows the user to increase contrast and resolution. Another important benefit is that a wide range of accessories were offered for the Nikon Alphaphot 2 series, some of which are still available (lenses, eyepieces, etc.).
As a result, the Alphaphot 2 can be equipped for a wide range of microscopy techniques, such as phase contrast, dark field, fluorescence, polarized light and epi-illumination microscopy.
Originally, using a trinocular phototube, the microscope could be configured for analogue photography in combination with Nikon Microflex FX series cameras. Optionally, it could also be coupled with analogue video (CCTV) cameras.
Several alternatives are available for connecting a digital camera to the Nikon Alphaphot 2. If the microscope has a phototube, this is the most convenient option for photographic documentation. We used a Canon EOS 650D in our tests. Of note, when attaching the camera to the phototube, the entire eyepiece head needs to be turned to the side.
The mounting option using the original Nikon phototube is depicted in the image on the left. In that case, only our DSLRCT LM digital adapter was needed to attach the camera to the microscope.
The mounting option that does not use the original Nikon phototube is shown in the image on the right. In this case, we used our TUST38C and DSLRCC LM digital adapter to attach the Canon EOS 650D to the microscope.
There is no optical difference between the two options.
Connecting a C-mount camera to the microscope is also a very simple process. We mounted a USB2 C-mount camera (in this case: the Imaging Source DFK 72BUC02 USB2 C-Mount) to the phototube. To do this, we used our TUST38C and our LM RelayLens05TC.
Since LM digital microscope adapters are not equipped with specific corrective optics (optical compensation), the image flaws associated with the microscope's lens also appear in the photographs taken with the camera. Tests we conducted using 10x lenses (10x/0.25x Ph1 DL) produced slight field curvatures around the edges, which are notable in the slightly blurred streaks towards the edges in the image below.
As with all older microscopes that have a finite optical system, the image quality of the Nikon Alphaphot 2 is average. However, truly excellent image quality can be achieved by using branded, infinity-corrected microscopes equipped with high-quality plan objective lenses. In these systems, residual image flaws are corrected by the elements inside the body of the microscope.
The Nikon Alphaphot 2 is a sound-quality laboratory microscope from the 1990s. It is also very suitable for use in schools. Connecting the microscope to camera systems for photographic documentation purposes is also a very simple process. The microscope’s image quality is average, a result that derives from its finite optical system design.
Fitting the microscope to digital single-lens reflex (DSLR), system or C-mount cameras is easy with our LM digital SLR adapters, which feature a plan achromatic optical system. Our products make it possible to capture top-quality microscope images. To help you select the adapter that is right for your camera, we have set up an online configurator on our website. You can also email us – ideally with attached photographs of your microscope.
Modern DSLR and system cameras offer the latest technology and are generally very well suited for microscopy applications. Most of them can be controlled remotely via PC/Mac. Because of their high sales volumes, they offer an excellent price/performance ratio compared to special-purpose microscope cameras.
Features of top DSLR and system cameras:
- Large, powerful full-frame sensors (36 x 24 mm)
- Sensor resolution of 50 megapixels
- High light sensitivity (ISO 100,000+)
- Extensive dynamic range (up to 14 aperture stops/f-stops)
- Short exposure times (1/8000 second):
- Full/Ultra HD (4k) video function
- Live video capture on external monitors in ultra HD quality
In most cases, these cameras are significantly more powerful than microscope cameras with smaller sensors (1/2" or 2/3"). On our website you will find our current camera recommendations and a camera ranking which is specifically tailored to microscopy applications.
Nikon D850 / Sony Alpha 7III / Sony Alpha 9 / Nikon D5 / Sony Alpha 7S II / Sony Alpha 7R II / Sony Alpha 7R III / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Nikon D610 / Nikon D600 / Canon EOS 6D / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 /
|Practical Test: Nikon D800|
|Testing report: Canon EOS 5D Mark III|
|LM photo microscope for C-mount camera|
|Microscope Digital Cameras: Camera ranking for microscopy use|
|Canon EOS 200D - practical test on microscope with LM DSLR widefield adapter with planachromatic optics|
|LM photo microscope with Nikon C-mount camera DS-Fi2 and control panel DS-L3 for measurement tasks, long-term studies and lengthy observations|
|LM microscope adapters: We’re impressed by the performance of Canon’s professional-grade EOS 5D Mark IV camera at the microscope!|
|Canon EOS 5D Mark II on the microscope|
|Controlling Olympus digital SLR cameras from your PC with the OLYMPUS Studio 2 software|
|The Sony Alpha 7R II – a top-notch camera with full-frame sensor for use in microscopy|
|Canon Rebel T4i (EOS 650D) in a practical test|
|Which digital camera works best on a microscope?|
|Convert your digital SLR camera into a professional microscope|
|Special mounting media for microscopy|
Tips and tricks to connect your digital camera and to process digital images