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Online configurator: LM microscope adapter for all digital cameras and microscopes
An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Microscope Adapter: LM wide-field adapter for eyepiece tubes with 30 mm inner diameter
LM digital SLR universal wide-field adapter: now with larger image field and focus settings
LM wide-field camcorder adapter for the new generation of ultra HD camcorders
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Macro Close-Up Lens: LM Macro lens 40/80 with plan achromatic optics
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Microscope Digital Cameras: Camera ranking for microscopy use
DSLR camera or special-purpose microscope cameras?

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Low budget 3D microscopy: how to create fascinating 3D transformations of a z-stack image series in microscopy and macroscopy

How can I visualise surface features such as wear and tear or surface roughness? How can I capture an image of an object under the microscope that makes it look as if it’s popping out from the screen?

In microscopy and macroscopy, focus stacking and the corresponding software tools are increasingly employed to extend the apparent depth of field of a photograph. Especially at higher magnifications of 30x, where, due to physical limits, the depth of field is very low, photographers often use focus stacking to improve depth of field. Over 100 separate photos are taken by gradually incrementing the focusing distance across the subject (this is the “z-stack”). With a specialised software, a composite image is then created, based on the sharpest regions from each of these separate photos.

We used a simple coin cell battery, available everywhere batteries are sold, for this process.

Helicon Focus 3D microfotography Box

These z-stacks can also be created by using a motorised focus rail (StackShot) and the Helicon Remote software. The finished z-stack, which contains more than 100 separate images, is then merged into one frame using the Helicon Fokus software. Because the image collection process is automated, all the images in the z-stack are captured with the same step size. It takes just a few mouse clicks and minutes of your time to create the finished image, which translates into significant improvements in workflow efficiency.

With our LM macroscopes and photomicroscopes, which are equipped with objective lenses that are optimised for this technique, it is possible to achieve the best possible image quality for each individual image. Achieving maximum resolution is a key aspect in the design of our devices.

LM makcroscope 24x with Stackshot Slider

The image above shows an LM macroscope with Cognisys StackShot system.

The LM macroscope has a central optical path and is thus considerably more powerful than comparable systems with a dual optical path or conventional stereomicroscopes. Thanks to its modular construction, it is extremely flexible and enables a multitude of magnification combinations (3x -> 18x), depending on which configuration of tube elements is used. The objective lenses are also available without a tripod.

Once the z-stack has been created, the rendering process can be started.

Helicon Focus 6.8. Rendering , 3d image

The data in the image file are then exported to create a 3D model with just one click.

Helicon Focus 3D rendering

The video above shows how a 3D model is created

Helicon’s “3D viewer” utility, which is included into the Helicon Focus installation, is designed to visualise 3D models. The object is displayed in a box, where it can be rotated and observed from any angle.

Helicon 3D data rotating

The 3D viewer supports almost all microscopic observation methods – bright field, dark field, incident light, fluorescence – at nearly any magnification.

Because DSLRs and system cameras have highly sophisticated camera sensors with up to 40 MP resolution and a huge dynamic range, it is possible to produce a highly accurate 3D dataset. We have compiled a set of camera recommendations to help you choose the DSLR that is best suited for your needs.


New LM Digital Adapter for:

Sony Alpha 9 / Canon EOS R with Canon Adapter EF-EOS R / Sony Alpha 7S II / Sony Alpha 7R III / Nikon Z6 with F-Mount Adapter FTZ / Sony Alpha 7R II / Nikon Z7 with F-Mount Adapter FTZ / Sony Alpha 7S / Nikon D850 / Canon EOS 1D X Mark II / Sony Alpha 7III / Nikon D5 / Canon EOS 1D X / Nikon D4s / Canon EOS 5D Mark IV / Nikon D4 / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Nikon D810 / Nikon D800 / Nikon D800E / Nikon Df / Panasonic Lumix DC-G9 / Nikon D610 / Nikon D600 / Canon EOS M50 / Canon EOS 6D / Sony Alpha 99 II (SLT-A99 II) / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Pentax K-1 Mark II / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Nikon D3x / Olympus OM-D E-M1 / Pentax KP / Nikon D3S /

Related Links
Test Canon EOS 4000D: Easy mounting on eyepiece- and photo tube with our LM microscope adapter
Nikon D850 fullframe-sensor camera for professionals offers enhanced capabilities for microscopy and macroscopy applications.
Sony Alpha 5100 (DSLM) on the microscope – easy connection to either phototube or eyepiece tube with the LM microscope adapter solutions
The Panasonic Lumix DC-G9 80 MP pro-grade camera and its performance in microscopy
Mirrorless system cameras Nikon Z6/ Z7: High-end USB microscope cameras with up to 45 MP sensor resolution
Test: Using the Canon EOS M50 on a microscope. Our LM microscope adapter solution allows for easy connection to either phototube or eyepiece tube
The Alpha 6500 is another top-end system camera from Sony that performs impressively on the microscope
The Pentax K-5 IIs DSLR camera tested on a microscope
Practical Test: Nikon D800
Testing report: Canon EOS 5D Mark III
The Canon Camera Connect app in microscopy: wireless communication between smartphone or tablet and Canon EOS cameras
Professional camera with full-frame sensor Leica M10 on the microscope
Microscope Digital Cameras: Camera ranking for microscopy use
The new Canon DSLR EOS 1300D – a microscope camera with an unbeatable price/performance ratio
Various LM digital adapter solutions for many microscopes with the new Canon EOS 77D DSLR
Canon EOS 200D - practical test on microscope with LM DSLR widefield adapter with planachromatic optics
Powerfull full-frame sensor microscope camera Canon EOS 6D Mark II for the sophisticated requirements of photomicrography
LM photo microscope for C-mount camera
LM photo microscope with Nikon C-mount camera DS-Fi2 and control panel DS-L3 for measurement tasks, long-term studies and lengthy observations
LM microscope adapters: We’re impressed by the performance of Canon’s professional-grade EOS 5D Mark IV camera at the microscope!
Canon EOS 5D Mark II on the microscope
Controlling Olympus digital SLR cameras from your PC with the OLYMPUS Studio 2 software

 

High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
Which digital camera works best on a microscope?
LM Makroskop 16x Convert your digital SLR camera into a professional microscope
Special mounting media  for microscopy

Tips and tricks to connect your digital camera and to process digital images
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 Demo Pictures

 
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