Micro Tech Lab Professional solutions for digital micro and macro photography
Products Reviews & buying guide Contact Order Sitemap References

 

An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Online configurator: LM microscope adapter for all digital cameras and microscopes
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Microscope Digital Cameras: Camera ranking for microscopy use
DSLR camera or special-purpose microscope cameras?
Zeiss Axiovert 200 – inverted microscopy at the highest level with digital system cameras and DSLR
High-quality adapter solution with integrated plan-achromatic optics for Zeiss OPMI microscopes with eyepiece tube
Test: Using the Canon EOS M50 on a microscope. Our LM microscope adapter solution allows for easy connection to either phototube or eyepiece tube
The Panasonic Lumix DC-G9 80 MP pro-grade camera and its performance in microscopy
Extreme macro photography:LM macroscope and a DSLR/DSLM camera

Folge Micro_Tech_Lab auf Facebook Folge Micro_Tech_Lab auf Twitter     179
Peripheral darkening (vignetting) in microscopy and macroscopy photography

Vignetting in micro or macrophotography is peripheral darkening at the edge of the image. This effect can be either desirable or undesirable depending on the field of application.

Vignetting is categorised according to different degrees of severity:


weak moderate severe

In addition, the symmetry of the vignetting is also judged. An asymmetrical appearance to the vignetting ring is the first indicator that the optical system is not correctly centred. Mostly the camera unit is not positioned perfectly over the microscope’s optical axis. In rare cases, the camera's sensor chip is not correctly positioned inside the camera body.In normal photography, these production tolerances mostly go unnoticed. However, when used in conjunction with a microscope, all mechanical irregularities and image distortions in the parallel light path are very much intensified.

Centric and non-centric position of the vignetting ring:

centric position non-centric position

Workaround: If it is not possible to adjust the optical system so that it is precisely centred, then with cameras with in-built zoom lenses it is possible to zoom in to the image until the vignetting and misalignment disappear. The image quality is not disadvantaged by this approach, as in the process any image section within the vignetting is enlarged to a full format image.

Depending on the field of application, vignetting may be acceptable. In the case of gastroscopic or endoscopic photography in medical use, 100% of the round image must be reproduced on the rectangular camera sensor. The doctor needs to view 100% of the information on the image transmitted through the fibre optics, and will therefore accept vignetting and blurring around the edges of the ring.

In microscopy, more importance is placed on achieving a full format, rectangular reproduction of the field of view than on achieving a circular reproduction of the field of view that is true to the original. The camera sensor should be illuminated in such a way that there is no possibility of vignetting. With this optical adjustment, the camera’s pixel count can be utilised to the optimum.

What actually causes vignetting?

The majority of optical elements such as lens elements, apertures, tubes and lens units are manufactured as round elements. Only the camera sensor is made as a rectangular electronic component. Vignetting is therefore the reproduction of the optical edge of the microscope’s light path. By reproducing the round microscope image on the rectangular camera sensor, all of the areas of the image lying outside of the round microscope image remain unlit and thus black.
Only with greater magnification of the round microscope image is the rectangular camera sensor exposed to light in such a way that the image is illuminated over the entire sensor area without peripheral darkening (vignetting).

If high image quality is important to you, then optical adjustment between the microscope and the camera is indispensable. The LM digital adapter ensures that the image is projected onto the camera chip at the optimum size. Furthermore, the microscope’s chromatic image distortions must be compensated for. As a result of the interaction between the optical limit and the light, there are considerably more serious image distortions on the edges than in the middle of the optical axis. If the adapter does not fulfil its function to the optimum, then there will be flaws in the reproduction (chromatic and spherical aberrations) and vignetting. An optimum adjustment between the microscope and the camera is only possible if the camera’s lens can be removed.
The camera lens unit with its many optical elements (up to 19 lens elements and more in the case of zoom lens units) only has the effect of reducing quality in the microscopic light path and is not needed in microphotography. For this reason, digital SLR cameras and professional camcorders are equipped with interchangeable lens mountings.
In this case, the microscope adapter is directly connected to the camera body via the bayonet mount.

The most common causes of vignetting in microphotography and macrophotography:
Related subjects

Compact cameras and camcorders on the microscope
DSLR cameras are simply better!



Caution! This manual is protected by copyright. Unauthorised reproduction thereof, in whole or in part, is punishable by law. Only use with MICRO TECH LABís approval.

New LM Digital Adapter for:

Sony Alpha 9 / Sony Alpha 7R III / Nikon Z6 with F-Mount Adapter FTZ / Sony Alpha 7R II / Nikon Z7 with F-Mount Adapter FTZ / Nikon D850 / Sony Alpha 7S II / Sony Alpha 7III / Nikon D5 / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Panasonic Lumix DC-G9 / Nikon D610 / Nikon D600 / Canon EOS M50 / Canon EOS 6D / Sony Alpha 99 II (SLT-A99 II) / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Pentax K-1 Mark II / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III /

Related Links

 

High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
Which digital camera works best on a microscope?
LM Makroskop 16x Convert your digital SLR camera into a professional microscope
Special mounting media  for microscopy

Tips and tricks to connect your digital camera and to process digital images
wing_drosophila

 

 Demo Pictures

 
© 1999-2018 MICRO TECH LAB. All rights reserved / Legal notice / Privacy policy / Newsletter / Press
 
--