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Full format sensor (24 × 36 mm) vs. half format sensor in microscopy

Large sensors provide in general a higher image quality. Especially colour depth and the signal/noise relatio is better with high quality sensors. With low-lighting (i.e. flourescence microscopy) when very little light reaches the sensor, it is of advantage if the sensor is large. Long exposure times and high ISO settings (> 200,000) make high demands on the camera.   But using full format sensors in microscopy does not have as many advantages as it does in conventional photography. In microscopy, however, the image quality depends very much on the type of microscope used.

Particularly at very high magnifications, the quality of the microscope also determines the resolution of the photographs. What limits the image resolution is exclusively the numerical aperture of the lens, and not the number of pixels. Microscopes are complex optical systems, and image improvements resulting from high numbers of pixels cannot be seen, as no new details in the structure can be reproduced through the resolution of the microscope optical system. From our standpoint, a camera’s zoom function in live view modeis considerably more important in microscopy than a high number of pixels. Using the live preview on the LCD display, the focus plane can be adjusted to its optimum setting. Also very important is that the camera can be navigated by PC or Mac (tethering).

For small magnifications of less than 10x, professional camera (with full format sensors) provide definitely better results.

Full format sensors mainly offer professional features  that are not offered by cheaper cameras (or only in part, depending on the pricing policy of the camera manufacturer). Among these are:

    * Live view
    * Computer control function (USB / LAN / WLAN), Canon EOS Utility, Nikon Remote Control
    * HDMI (high resolution video output)
    * Pivoting LCD display
    * High number of pixels, currently 36 megapixels
    * Excellent signal-to-noise-ratio of the sensor chip with high ISO settings
    * Greater colour depth – 14 or 16 bits per channel RGB (Red/Green/Blue)
    * Ability to take a series of images (at present, approx. 10 images per second)
    * Low shutter release delay (in the millisecond range)
    * Low-vibration plane shutter 
    * Fast exposure time (1/8,000)
    * Long exposure times


The use of highly corrected plan fluoride or plan apochromatic microscope lenses is highly recommended. These are, however, very expensive, starting at around EUR 1,000. For about the same price, you can purchase a digital SLR camera and a suitable LM digital adapter. Digital full format SLR cameras are available for around EUR 1,800 or more, and for a good microscope you will pay from EUR 10,000 and up.

For the following fullframe cameras you will find testing reports on our website: Nikon D4, Nikon D800, Nikon D610, Nikon Df, Canon EOS-1D X, Canon EOS 6D, Canon EOS 5D Mark II, Canon EOS 5D Mark III


 

Update: 15.07.2014



Caution! This manual is protected by copyright. Unauthorised reproduction thereof, in whole or in part, is punishable by law. Only use with MICRO TECH LAB’s approval.

New LM Digital Adapter for: Nikon Z9 / Sony Alpha 1 / Sony Alpha 9 II (ILCE-9M2) / Sony Alpha 9 / Nikon D6 / Sony Alpha 7R IV / Canon EOS R5 / Sony Alpha 7S II / Sony Alpha 7R III / Canon EOS R6 / Nikon Z6 / Nikon Z6II / Sony Alpha 7R II / Nikon Z7 / Nikon Z7II / Canon EOS R / Canon EOS Ra (Astro) / Nikon Z5 / Sony Alpha 7C / Canon EOS RP / Sony Alpha 7S / Canon EOS 1D X Mark III / Nikon Z50 / Nikon D850 / Canon EOS 1D X Mark II / Nikon D780 / Sony Alpha 7III / Nikon D5 / Sony Alpha 6600 / Sony Alpha 6400 / Sony Alpha 6100 / Canon EOS 1D X / Nikon D4s / Olympus OM-D E-M5 III / Canon EOS 90D / Canon EOS 5D Mark IV / Nikon D4 / Nikon D750 / Canon EOS 6D Mark II / Fujifilm X-T4 / Fujifilm X-T3 /