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An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Online configurator: LM microscope adapter for all digital cameras and microscopes
LM adapter solution for c-mount microscope ports with reduction optics (0.5x / 0.6x / 0.7x or f=55mm / f=75mm / f=100mm)
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Macro Close-Up Lens: LM Macro lens 40/80 with plan achromatic optics
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Service: improving image quality by giving your microscope a professional check-up
Camera ranking for microscopy use
Camera recommendation for microscopy application
DSLR camera or special-purpose microscope cameras?
Image sensor: dynamic range and it's influence on image quality
Microsocope recommendation
The Zeiss Stemi 508 stereo microscope: microscope adapters for digital cameras (DLSR and system cameras)
The Olympus IX2 series: enhanced versatility for use in a wide range of application
Our LM digital adapters open the Zeiss SV8 stereo microscope to a new, digital world
Nikon’s SMZ 645 and 660 stereo microscopes in microphotography
With the help of LM Digital adapter the inverse Nikon Eclipse MA100 and MA200 microscope become fit for photomicrography
Capture exceptional images with the Leica DM4 B/DM6 B and our LM digital adapters
LM photo microscope with Nikon C-mount camera DS-Fi2 and control panel DS-L3 for measurement tasks, long-term studies and lengthy observations
Install modern camera systems (DSLR, system- or c-mount cameras) with our LM digital adapter on the Olympus SZX9 – a stereo microscope for industrial applications
Capture One Pro software: Tethering in microscopy with Live View for a large variety of cameras
Preview: The Nikon D850 – the new number 1 in our camera ranking!
Sony Alpha 9 – a camera that has everything you need!
Sony Alpha 7S II – a video specialist with ultra-high light sensitivity
Panasonic Lumix DC-GH5 – an interesting solution for long-term video recording at the microscope
Professional camera with full-frame sensor Leica M10 on the microscope
The new Canon DSLR EOS 1300D – a “microscope camera” with an unbeatable price/performance ratio

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Micro-Tech-Lab  Newsletter from 20.11.2008

  The december issue of our newsletter is dedicated to the topic of photography through a microscope. After receiving an increasing number of enquiries from our customers, we decided that it would be useful to shed some light on choosing a suitable camera for taking images using a microscope.  

Using full-frame digital SLR cameras in microscopy
 

The use of large sensors in microscopy does not have as many advantages as it does in conventional photography. Having a high number of pixels is also not hugely significant, as the image resolution is exclusively determined by the numeric aperture of the lens. Other camera features, on the other hand, such as colour depth, signal to noise ratio and light sensitivity, are far more important.
A whole range of camera components are responsible for creating high-quality microscope photos. Therefore it is important that each of these components is of high quality, as the entire chain can only be as strong as its weakest link. This signal chain consists of a sensor, an amplifier, an analogue-to-digital converter (ADC) and a digital signal processor (DSP).
 

Picture 1: Signal chain

View Image

When the photon strikes the sensor chip, it is stored as an electrical charge. This electrical charge is proportional to the number of photons present. The analogue voltage is increased by the amplifier before it reaches the analogue-to-digital converter (ADC). Only now does the information exist in digital form. Next, the digital signal processor (DSP) optimises the colour reproduction, and finally the data stream is processed for storage on the flash memory card (CF/MMD card).
As a rule, full-frame digital SLR cameras contain only top-quality components, and for this reason they tend to fall into the higher price bracket. Professional features such as remote control, mirror lock-up, LiveView and HDMI output generally come as standard on these models.

In addition, the colour depth of each colour channel is also important. Each basic colour (red/green/blue ==> RGB) has a resolution of between 12 and 16 bits. The higher this value, the more precise the reproduction of nuances in colour. If this number is multiplied by three (for each of the three basic colours) the result is the total memory depth of the colours that will be reproduced. At a resolution of 14 bits per channel, the result is a colour depth of 42 bits. In comparison, cheap compact cameras have only a 24 bit colour depth. The uncompressed data is stored in RAW format. Since this format has a larger storage requirement, users usually select the JPG format instead, which means that a lot of information is lost. Depending on the JPG image’s level of compression, a choice has to be made between storage space reduction and quality.

Overview of some cameras

   

     

 

Format (mm)

Colour depth (bits)

ADC
(bits)

HDMI

LiveView

Number of pixels (megapixels)

Nikon D4 24 x 36 42 14 Yes Yes 16

Nikon D3

24 x 36

42

14 

Yes

Yes

12

Nikon D300

23.6 x 15.8

42

14 

Yes

Yes

13

Nikon D7000 23.6 x 15.6 36 12 Yes Yes 16.2
Nikon D800 24 x 36 42 14 Yes Yes 36
Canon EOS 5D Mark II 24 x 36 42 14 Yes Yes 22

Canon EOS 5D Mark II

24 x 36

42

14 

Yes

Yes

21

Canon EOS 650D 22.3 x 14.9 42 14 Yes Yes 18
Canon EOS 600D 22.3 x 14.9 42 14 Yes Yes 18

Canon EOS 1000D

22.2 x 14.8

42

14

No

Yes

10

Olympus E3

17.3 x 13

36

12

No

Yes

10

Olympus E-520

17.3 x 13

36

12

No

Yes

1


 For professional use in microscopy, we recommend high-quality full-frame digital SLR cameras, particularly in the case of special processes (e.g. fluorescence microscopy). In addition, we offer special LM digital adapters for full-frame sensors, which guarantee optimum illumination. To help make your choice easier, we have created a special page on our website:   



Conclusion:

If microscope photos of extraordinary high quality are important to you, then you should always choose a digital SLR camera with a full-frame sensor. It should be mentioned, however, that cameras with a half-frame sensor or a four-thirds chip can also provide very good results. Here, it is particularly important that the camera used is equipped with a LiveView function and mirror lock-up, as only this will enable you to achieve the optimum level of focus that you require.

A preview of issue 3 of our newsletter:


New LM Digital Adapter for:

Nikon D850 / Sony Alpha 9 / Nikon D5 / Sony Alpha 7S II / Sony Alpha 7R II / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Nikon D610 / Nikon D600 / Canon EOS 6D / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Nikon D3x / Olympus OM-D E-M1 / Nikon D3S / Canon EOS 750D / Rebel T6i /

Related Links

 

High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
Which digital camera works best on a microscope?
LM Makroskop 16x Convert your digital SLR camera into a professional microscope
Special mounting media  for microscopy

Tips and tricks to connect your digital camera and to process digital images
wing_drosophila

 

 Demo Pictures

 
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