Use of Nikon’s entry-level single lens reflex (DSLR) cameras at the microscope
Nikon economises with the automatic exposure control!
With the lower-priced Nikon D40 /
D40x / D50 / D60 / D90 / D100 / D5000 models, only limited
exposure is possible without the original lens. The automatic exposure
control only works with Nikon digital lenses.
If you wish to use an analogue lens, camera adapter or bellows, the
camera housing will not allow you to control the automatic exposure in
manual mode. Only with the histogram display, or even more laboriously
with exposure bracketing, is it possible to determine the ideal
exposure time. The automatic functions such as automatic aperture
priority or automatic exposure time calculation are not possible
without the original AF lens being in place.
Especially in use with a telescope or microscope, where the microscope
adapter is fitted directly to the camera body by means of a T2 bayonet
fitting, this is a considerable disadvantage.
The better Nikon models D200 / D300 / D700 / D3 / D3x also have a fully
automatic exposure control function without the original AF lens. Even
without the original lens being fitted or when using the camera at the
microscope, the camera body carries out an automatic exposure.
Conclusion: If you want to use a Nikon camera with a microscope, then you should make sure that you use a “better” Nikon model.
Perfect automatic exposure control is very important for microscopy.
Other manufacturers, such as Canon or Olympus, even equip their
entry-level cameras with fully functioning automatic exposure control
for use without camera lenses.
Which digital camera functions best on a microscope?
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