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An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Online configurator: LM microscope adapter for all digital cameras and microscopes
LM adapter solution for c-mount microscope ports with reduction optics (0.5x / 0.6x / 0.7x or f=55mm / f=75mm / f=100mm)
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Macro Close-Up Lens: LM Macro lens 40/80 with plan achromatic optics
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Service: improving image quality by giving your microscope a professional check-up
Camera ranking for microscopy use
Camera recommendation for microscopy application
DSLR camera or special-purpose microscope cameras?
Image sensor: dynamic range and it's influence on image quality
Microsocope recommendation
The Zeiss Stemi 508 stereo microscope: microscope adapters for digital cameras (DLSR and system cameras)
The Olympus IX2 series: enhanced versatility for use in a wide range of application
Our LM digital adapters open the Zeiss SV8 stereo microscope to a new, digital world
Nikon’s SMZ 645 and 660 stereo microscopes in microphotography
With the help of LM Digital adapter the inverse Nikon Eclipse MA100 and MA200 microscope become fit for photomicrography
Capture exceptional images with the Leica DM4 B/DM6 B and our LM digital adapters
LM photo microscope with Nikon C-mount camera DS-Fi2 and control panel DS-L3 for measurement tasks, long-term studies and lengthy observations
Install modern camera systems (DSLR, system- or c-mount cameras) with our LM digital adapter on the Olympus SZX9 – a stereo microscope for industrial applications
Capture One Pro software: Tethering in microscopy with Live View for a large variety of cameras
Preview: The Nikon D850 – the new number 1 in our camera ranking!
Sony Alpha 9 – a camera that has everything you need!
Sony Alpha 7S II – a video specialist with ultra-high light sensitivity
Panasonic Lumix DC-GH5 – an interesting solution for long-term video recording at the microscope
Professional camera with full-frame sensor Leica M10 on the microscope
The new Canon DSLR EOS 1300D – a “microscope camera” with an unbeatable price/performance ratio

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Peripheral darkening (vignetting) in microscopy and macroscopy photography

Vignetting in micro or macrophotography is peripheral darkening at the edge of the image. This effect can be either desirable or undesirable depending on the field of application.

Vignetting is categorised according to different degrees of severity:


weak moderate severe

In addition, the symmetry of the vignetting is also judged. An asymmetrical appearance to the vignetting ring is the first indicator that the optical system is not correctly centred. Mostly the camera unit is not positioned perfectly over the microscope’s optical axis. In rare cases, the camera's sensor chip is not correctly positioned inside the camera body.In normal photography, these production tolerances mostly go unnoticed. However, when used in conjunction with a microscope, all mechanical irregularities and image distortions in the parallel light path are very much intensified.

Centric and non-centric position of the vignetting ring:

centric position non-centric position

Workaround: If it is not possible to adjust the optical system so that it is precisely centred, then with cameras with in-built zoom lenses it is possible to zoom in to the image until the vignetting and misalignment disappear. The image quality is not disadvantaged by this approach, as in the process any image section within the vignetting is enlarged to a full format image.

Depending on the field of application, vignetting may be acceptable. In the case of gastroscopic or endoscopic photography in medical use, 100% of the round image must be reproduced on the rectangular camera sensor. The doctor needs to view 100% of the information on the image transmitted through the fibre optics, and will therefore accept vignetting and blurring around the edges of the ring.

In microscopy, more importance is placed on achieving a full format, rectangular reproduction of the field of view than on achieving a circular reproduction of the field of view that is true to the original. The camera sensor should be illuminated in such a way that there is no possibility of vignetting. With this optical adjustment, the camera’s pixel count can be utilised to the optimum.

What actually causes vignetting?

The majority of optical elements such as lens elements, apertures, tubes and lens units are manufactured as round elements. Only the camera sensor is made as a rectangular electronic component. Vignetting is therefore the reproduction of the optical edge of the microscope’s light path. By reproducing the round microscope image on the rectangular camera sensor, all of the areas of the image lying outside of the round microscope image remain unlit and thus black.
Only with greater magnification of the round microscope image is the rectangular camera sensor exposed to light in such a way that the image is illuminated over the entire sensor area without peripheral darkening (vignetting).

If high image quality is important to you, then optical adjustment between the microscope and the camera is indispensable. The LM digital adapter ensures that the image is projected onto the camera chip at the optimum size. Furthermore, the microscope’s chromatic image distortions must be compensated for. As a result of the interaction between the optical limit and the light, there are considerably more serious image distortions on the edges than in the middle of the optical axis. If the adapter does not fulfil its function to the optimum, then there will be flaws in the reproduction (chromatic and spherical aberrations) and vignetting. An optimum adjustment between the microscope and the camera is only possible if the camera’s lens can be removed.
The camera lens unit with its many optical elements (up to 19 lens elements and more in the case of zoom lens units) only has the effect of reducing quality in the microscopic light path and is not needed in microphotography. For this reason, digital SLR cameras and professional camcorders are equipped with interchangeable lens mountings.
In this case, the microscope adapter is directly connected to the camera body via the bayonet mount.

The most common causes of vignetting in microphotography and macrophotography:
Related subjects

Compact cameras and camcorders on the microscope
DSLR cameras are simply better!



Caution! This manual is protected by copyright. Unauthorised reproduction thereof, in whole or in part, is punishable by law. Only use with MICRO TECH LAB’s approval.

New LM Digital Adapter for:

Nikon D850 / Sony Alpha 9 / Nikon D5 / Sony Alpha 7S II / Sony Alpha 7R II / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Nikon D610 / Nikon D600 / Canon EOS 6D / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Nikon D3x / Olympus OM-D E-M1 / Nikon D3S / Canon EOS 750D / Rebel T6i /

Related Links

 

High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
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