Micro Tech Lab Professional solutions for digital micro and macro photography
Products Reviews & buying guide Contact Order Sitemap References

 

An overview of the LM microscope adapters
We offer LM digital adapter solutions for the following microscopes
Online configurator: LM microscope adapter for all digital cameras and microscopes
LM adapter solution for c-mount microscope ports with reduction optics (0.5x / 0.6x / 0.7x or f=55mm / f=75mm / f=100mm)
LM Macroscope 24x (15x and 11x) for Focus Stacking: Highest resolution without compromise
LM Macroscope 9x (5x and 3x) for Focus Stacking: Highest resolution without compromise
LM photo microscopes: the flexible photography solution for large sensor cameras!
Macro Close-Up Lens: LM Macro lens 40/80 with plan achromatic optics
Special mounting medium for microscopy, non toxic,water solved,light hardening,fast solidifying and drying,neutraldoes not make air bubbles,high optical solution,color protecting,solvent free,high refractive index
Micro Tech Lab advisory service: microscopy, digital cameras, high quality photo
Service: improving image quality by giving your microscope a professional check-up
Camera ranking for microscopy use
Camera recommendation for microscopy application
DSLR camera or special-purpose microscope cameras?
Image sensor: dynamic range and it's influence on image quality
Microsocope recommendation
The Zeiss Stemi 508 stereo microscope: microscope adapters for digital cameras (DLSR and system cameras)
The Olympus IX2 series: enhanced versatility for use in a wide range of application
Our LM digital adapters open the Zeiss SV8 stereo microscope to a new, digital world
Nikon’s SMZ 645 and 660 stereo microscopes in microphotography
With the help of LM Digital adapter the inverse Nikon Eclipse MA100 and MA200 microscope become fit for photomicrography
Capture exceptional images with the Leica DM4 B/DM6 B and our LM digital adapters
LM photo microscope with Nikon C-mount camera DS-Fi2 and control panel DS-L3 for measurement tasks, long-term studies and lengthy observations
Install modern camera systems (DSLR, system- or c-mount cameras) with our LM digital adapter on the Olympus SZX9 – a stereo microscope for industrial applications
Capture One Pro software: Tethering in microscopy with Live View for a large variety of cameras
Preview: The Nikon D850 – the new number 1 in our camera ranking!
Sony Alpha 9 – a camera that has everything you need!
Sony Alpha 7S II – a video specialist with ultra-high light sensitivity
Panasonic Lumix DC-GH5 – an interesting solution for long-term video recording at the microscope
Professional camera with full-frame sensor Leica M10 on the microscope

Folge Micro_Tech_Lab auf Facebook Folge Micro_Tech_Lab auf Twitter     258
Sensor sensitivity (ISO) in digital cameras

The ISO value indicates a camera sensor’s sensitivity to light. The lower the ISO number, the less sensitive the sensor is to light. High ISO values, on the other hand, mean that quality images can be created even in low light conditions. In analogue photography, the ISO specification denotes the photosensitivity of photographic plates and films, which undergo a chemical reaction when they come into contact with light. Photographers working in analogue should select the photosensitivity of their film in advance, before starting a project, since it is tricky to change films while working. With digital cameras, there are no chemical changes to the sensor and the ISO sensitivity can be set differently for each picture. When light hits the sensor (semiconductor chip), an electrical charge proportional to the amount of light is released and stored. This is called the photoelectric effect (electrons are emitted by a semiconductor exposed to light). At the end of the exposure process this voltage (electrical charge) is read by the sensor and processed

ISO settings have a direct influence on the amplifier before the analogue-to-digital converter (ADC): high ISO numbers give more amplification, while low ISO numbers give less amplification. The camera sensor itself always maintains the same degree of sensitivity; only the level of amplification changes. Depending on lighting conditions, the ISO setting will either be selected automatically by the camera or can be changed manually.




The drawback of increased light sensitivity is an increase in noise – the image loses contrast and appears blurry. Noise levels are strongly influenced by the quality of the camera used. High ISO settings are therefore only advisable with high-quality sensors, in order to avoid excessive image noise. In recent years, semiconductor manufacturing has become more advanced, and this has improved signal-to-noise ratios.
 
A large pixel area (large sensor) can capture light quanta more effectively. In recent years, however, camera manufacturers have tended more and more frequently to reduce the size of the pixel area. This is detrimental to the signal-to-noise ratio. For each camera model, depending on the production technology involved, there is an optimal relationship between the number of pixels and the signal-to-noise ratio: this is determined by the camera’s sensor size. Camera manufacturers have started to notice this and turned their attention to achieving a better signal-to-noise ratio and better colour rendition (e.g. Canon EOS 1DX, Canon C300, etc.). In some models, tiny micro-lenses are positioned above each pixel site in order to capture the light more effectively. 
 
The resolution capability (colour depth) of the analogue-to-digital converter (ADC) is a key factor in determining image quality. Colour depth describes the maximum number of colours available to represent a single pixel in an image. For the three basic colour channels (RGB, red/green/blue), an analogue-to-digital conversion is carried out separately per channel. At present, resolutions between 12 and 16 bits per channel are used. In 14-bit mode, the total colour depth is 42 bits for all three channels.
 
Table listing the current top cameras:
 
Camera
Sensitivity [ISO] Colour depht (Bit)
Nikon D3x 100   -   1.600
42
Nikon D3s 200   - 12.800
42
Nikon D4 100 - 12.800 42
Nikon D800 100 - 6.400 42
Canon EOS 600D 100   -   6.400
42
Canon  EOS 60D 100   -   3.200
42
Canon EOS 1D X 100  -  51.200 
42
Canon EOS 1D Mark IV 100   - 12.800
42
Canon EOS 5D Mark III 100 - 12.800 42
Canon EOS 650D 100 - 6.400 42
Olympus E5 100   -   6.400
36
 
After the signal has been converted in the ADC, the image processer further reduces noise. If digital noise reduction is applied to aggressively, image information may be lost.
Conclusion:

The ISO value indicates a camera sensor’s sensitivity to light. The lower the ISO number, the less sensitive the sensor is to light. High ISO settings allow quality images to be taken even when light conditions are poor. However, the drawback of increased light sensitivity is an increase in noise: the image becomes grainy and lacks detail. Therefore, it is only advisable to select high ISO numbers when using high-quality camera sensors, with high colour depth and a large signal-to-noise ratio.

Update: 06.07.2012


New LM Digital Adapter for:

Nikon D850 / Sony Alpha 9 / Nikon D5 / Sony Alpha 7S II / Sony Alpha 7R II / Sony Alpha 7S / Canon EOS 5D Mark IV / Nikon D750 / Canon EOS 6D Mark II / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Canon EOS 1D X / Nikon D810 / Nikon D4s / Nikon D800 / Nikon D800E / Nikon D4 / Nikon Df / Nikon D610 / Nikon D600 / Canon EOS 6D / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II / Canon EOS 80D / Canon EOS 5DS / Sony Alpha 77 II / Canon EOS 70D / Nikon D7200 / Canon EOS 200D / Canon EOS 800D / Rebel T7i / Canon EOS 77D / Canon EOS 5D Mark III / Canon EOS 60D / Sony Alpha 7R / Sony Alpha 7 / Nikon DS-Qi2 (Microscope Camera) / Nikon D3x / Olympus OM-D E-M1 / Nikon D3S / Canon EOS 750D / Rebel T6i /

Related Links
Professional camera with full-frame sensor Leica M10 on the microscope
Sony Alpha 9 – a camera that has everything you need!
Preview: The Nikon D850 – the new number 1 in our camera ranking!
Sony Alpha 7S II – a video specialist with ultra-high light sensitivity
Tested for you: Canon EOS M5 system camera with a microscope
LM microscope adapters: Canon’s new midrange DSLR camera, the EOS 80 D, is a perfect fit for microscopes!
Canon EOS Utility 2 and Apple computer
Canon EOS 5D Mark II on the microscope
Taking photos with the Nikon SMZ18, SMZ25, SMZ800N and SMZ1270i stereo microscopes using our tried and tested LM digital adapters
Canon Rebel T4i (EOS 650D) in a practical test
Improved picture quality through mirror lock-up in SLR cameras
Macro Close-Up Lens: LM Macro lens 40/80 with plan achromatic optics
Improving the quality of your microscope images
Camera recommendation for microscopy application
Canon Utility Software 3
Focus stacking with our LM macroscopes and LM photo microscopes
LM photo microscopes: the flexible photography solution for large sensor cameras!
Sensor heating in Nikon DSLR cameras in Live View mode
DSLR camera or special-purpose microscope cameras?
All in one: LM Digital SLR Universal Adapter for phototube and the eyepiece tube (C-Mount, 23.2mm, 30mm 37mm, 38mm, 42mm)
In microscopy, digital SLR cameras are the better video cameras!
PC remote control of the Nikon Coolpix digital camera in microscopy
Controlling Olympus digital SLR cameras from your PC with the OLYMPUS Studio 2 software
An overview of the LM microscope adapters
Using HD / UHD / 4k / 6k components in microphotography
Using a tablet (Toshiba runnin Windows 8.1.) for photomicrography
Which camera types are of interest for use in microscopy?
A useful item – battery adapters for DSLR cameras
Testing report: Nikon Camera Control Pro2
Microscope or Macroscope Lighting: 2 IKEA-LED work lamps JANSJÖ for € 9.99 each
Digital macro photography with “classic analogue lenses” at bargain prices
Photographing through a microscope eyepiece
Live video streaming and video capture with a mobile computer (notebook)
Controlling cameras remotely via PC or Mac
Time lapse shots with the Canon Utility software – using the timer function
Tethering software Sony
Image sensor: dynamic range and it's influence on image quality
The camera shutter - In photography, a shutter is used to control exposure time. It has a significant influence on image quality.
The Nikon D810 full frame camera in microscopy
Olympus Capture Softwore use in microscopy application
Nikon D7100 on the microscope
Canon EOS remote app: operating EOS cameras by remote control via iPad and smartphone

 

High-end intermediate optics for connecting microscopes to:
  • digital SLR cameras
  • digital mirrorless system cameras with an interchangeable lens mount
  • c-mount-, USB- and firewire cameras
  • digital compact cameras and camcorders
[Further information amd prices]
 
Which digital camera works best on a microscope?
LM Makroskop 16x Convert your digital SLR camera into a professional microscope
Special mounting media  for microscopy

Tips and tricks to connect your digital camera and to process digital images
wing_drosophila

 

 Demo Pictures

 
© 1999-2016 MICRO TECH LAB. All rights reserved / Legal notice / Privacy policy / Newsletter / Press
 
OK